Part:BBa_K199000:Design

CCCUC tRNA Suppressor (Produces Serine)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 38
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We used the 9 base anticodon loop that is complementary to the 5 base pair codon CCCUC.

Source

De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)